In this study, the chronic effects of 2 BZT-UVs, 2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (UV-234) and 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV-328) were evaluated for each compound alone and in a mixture on the freshwater green algae Chlamydomonas reinhardtii and the freshwater crustacean Daphnia magna. Life-history endpoints (viability, reproduction, and growth) and oxidative stress–related biomarkers (gene transcription, reactive oxygen species [ROS] production, and lipid peroxidation) were measured. Results have been published in a peer-reviewed scientific article. Citation: M. Giraudo, G. Cottin, M. Esperanza, P. Gagnon, A.O. De SIlva, M. Houde. 2017. Transcriptional and cellular effects of benzotriazole UV stabilizers UV-234 and UV-328 in the freshwater invertebrates Chlamydomonas reinhardtii and Daphnia magna. Environmental Toxicology and Chemistry 36(12): 3333-3342. doi.org/10.1002/etc.3908

In this study, Daphnia magna were exposed over three 21d generations to an environmentally relevant concentration of TBOEP (10 μg/L) and effects were evaluated at the gene transcription, protein, and life-history (i.e., survival, reproduction and growth) levels. Results were published in a scientific article that can be found here: https://doi.org/10.1016/j.aquatox.2017.07.003

The transcriptomic response of Daphnia magna exposed to sublethal doses of 1H-benzotriazole (BTR), 5-methyl-1H-benzotriazole, (5MeBTR), and 5-chloro-1H-benzotriazole (5ClBTR) was evaluated using RNA-sequencing and quantitative real-time PCR. Cellular (chitinase activity) and life-history endpoints (survival, number of neonates, growth) were also investigated. Results have been published in a scientific article that can be found here: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171763

Two precipitation measurement super-sites were established in Saskatchewan in anticipation of the World Meteorological Organization (WMO) intercomparison initiatives for automated in-situ measurement of solid precipitation and snow (2013-2015) and to provide infrastructure support for exploring national solid precipitation measurement issues. The Bratt’s Lake site is in the central Canadian prairies approximately 30 km south of Regina. The Caribou Creek site is located in the southern Boreal forest approximately 100 km Northeast of Prince Albert. Both sites contributed to the WMO Solid Precipitation Inter-Comparison Experiment (SPICE). The objectives of these intercomparison sites are to characterize and provide guidance on the behaviour and performance of automated systems for the in-situ measurement of solid precipitation. Each of the sites provides a different perspective for measuring precipitation. Caribou Creek is characterized by a relatively longer and colder winter season but experiences lower wind speeds during precipitation due to less exposure. The Bratt’s Lake site is drier with a shorter snow accumulation season but experiences relatively high wind speeds due to high exposure, making accurate measurement of solid precipitation more problematic. The data presented here generally spans between the beginning of October and the end of April starting in the fall of 2013 (the first season of WMO-SPICE). The focus is on hourly accumulations of precipitation measured using various configurations of automated instruments and wind shields, including a WMO Double Fence Automated Reference (DFAR) measurement. Also included are matching ancillary data (wind speed and temperature) corresponding to the same measurement periods as precipitation. A more detailed description of the site measurements is included in the documentation residing in the repository. A subset of this data (2013-2017 cold seasons) was previously published in the ECCC catalogue at a 30-min temporal resolution and can be found at https://doi.org/10.18164/63773b5b-5529-4b1e-9150-10acb84d59f0 with a further description via Smith et al. (2019; https://doi.org/10.5194/essd-11-1337-2019). Unlike the previous published dataset, this publication will consist of a larger number of instrument configurations and is intended to be updated annually.

In this study, Daphnia magna transcriptomic response was measured by microarray to evaluate sublethal effects of TBOEP as part of a multi-level biological approach including specific gene transcription measured by qRT-PCR, enzyme activity, and life-history endpoints (i.e., survival, growth, and reproduction). Data are a part subject of a publication containing method details, full QA/QC, interpretation and conclusions. Citation: M. Giraudo, M. Douville, M. Houde. 2015. Chronic toxicity evaluation of the flame retardant tris (2-butoxyethyl) phosphate (TBOEP) using Daphnia magna transcriptomic response. Chemosphere 132: 19-165. https://doi.org/10.1016/j.chemosphere.2015.03.028

In this study, the chronic effects of two emerging brominated flame retardants, 1,2-bis(2,4,6-tribromophenoxy)ethane(BTBPE) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB) were evaluated in diet exposed juvenile rainbow trout (Oncorhynchus mykiss). Fish uptake was measured after 28-d of exposure. Liver gene transcription analysis using RNA-sequencing was also performed, complemented by qRT-PCR to measure transcription levels of genes related to the immune response in the liver and the head kidney. Lysozyme enzymatic activity was also measured in head kidneys of exposed fish. Results are published in a peer-reviewed scientific publication. Citation: Giraudo, M., Douville, M., Letcher, R. J., & Houde, M. (2017). Effects of food-borne exposure of juvenile rainbow trout (Oncorhynchus mykiss) to emerging brominated flame retardants 1,2-bis(2,4,6-tribromophenoxy)ethane and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate. Aquatic Toxicology (Amsterdam, Netherlands), 186, 40-49. doi.org/10.1016/j.aquatox.2017.02.023

The objectives of the present study were to evaluate the chronixc effects of 2 BZT‐UVs alone and in a mixture, 2‐(2H‐benzotriazol‐2‐yl)‐4,6‐bis(1‐methyl‐1‐phenylethyl)phenol (UV‐234) and 2‐(2H‐benzotriazol‐2‐yl)‐4,6‐di‐tert‐pentylphenol (UV‐328), in juvenile rainbow trout (Oncorhynchus mykiss) chronically exposed (for 28 d) through the diet. Chemical analyses were used to measure liver accumulation of the 2 compounds in exposed trout. Hepatic RNA‐sequencing and qRT-PCR were used to measure gene transcription levels in exposed fish. Results were published in a scientific article that can be found here: https://doi.org/10.1002/etc.4676