In this study, Daphnia magna were exposed over three 21d generations to an environmentally relevant concentration of TBOEP (10 μg/L) and effects were evaluated at the gene transcription, protein, and life-history (i.e., survival, reproduction and growth) levels. Results were published in a scientific article that can be found here: https://doi.org/10.1016/j.aquatox.2017.07.003

The transcriptomic response of Daphnia magna exposed to sublethal doses of 1H-benzotriazole (BTR), 5-methyl-1H-benzotriazole, (5MeBTR), and 5-chloro-1H-benzotriazole (5ClBTR) was evaluated using RNA-sequencing and quantitative real-time PCR. Cellular (chitinase activity) and life-history endpoints (survival, number of neonates, growth) were also investigated. Results have been published in a scientific article that can be found here: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171763

In this study, the chronic effects of 2 BZT-UVs, 2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (UV-234) and 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV-328) were evaluated for each compound alone and in a mixture on the freshwater green algae Chlamydomonas reinhardtii and the freshwater crustacean Daphnia magna. Life-history endpoints (viability, reproduction, and growth) and oxidative stress–related biomarkers (gene transcription, reactive oxygen species [ROS] production, and lipid peroxidation) were measured. Results have been published in a peer-reviewed scientific article. Citation: M. Giraudo, G. Cottin, M. Esperanza, P. Gagnon, A.O. De SIlva, M. Houde. 2017. Transcriptional and cellular effects of benzotriazole UV stabilizers UV-234 and UV-328 in the freshwater invertebrates Chlamydomonas reinhardtii and Daphnia magna. Environmental Toxicology and Chemistry 36(12): 3333-3342. doi.org/10.1002/etc.3908

In this study, Daphnia magna transcriptomic response was measured by microarray to evaluate sublethal effects of TBOEP as part of a multi-level biological approach including specific gene transcription measured by qRT-PCR, enzyme activity, and life-history endpoints (i.e., survival, growth, and reproduction). Data are a part subject of a publication containing method details, full QA/QC, interpretation and conclusions. Citation: M. Giraudo, M. Douville, M. Houde. 2015. Chronic toxicity evaluation of the flame retardant tris (2-butoxyethyl) phosphate (TBOEP) using Daphnia magna transcriptomic response. Chemosphere 132: 19-165. https://doi.org/10.1016/j.chemosphere.2015.03.028

In this study, the chronic effects of two emerging brominated flame retardants, 1,2-bis(2,4,6-tribromophenoxy)ethane(BTBPE) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB) were evaluated in diet exposed juvenile rainbow trout (Oncorhynchus mykiss). Fish uptake was measured after 28-d of exposure. Liver gene transcription analysis using RNA-sequencing was also performed, complemented by qRT-PCR to measure transcription levels of genes related to the immune response in the liver and the head kidney. Lysozyme enzymatic activity was also measured in head kidneys of exposed fish. Results are published in a peer-reviewed scientific publication. Citation: Giraudo, M., Douville, M., Letcher, R. J., & Houde, M. (2017). Effects of food-borne exposure of juvenile rainbow trout (Oncorhynchus mykiss) to emerging brominated flame retardants 1,2-bis(2,4,6-tribromophenoxy)ethane and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate. Aquatic Toxicology (Amsterdam, Netherlands), 186, 40-49. doi.org/10.1016/j.aquatox.2017.02.023

The objectives of the present study were to evaluate the chronixc effects of 2 BZT‐UVs alone and in a mixture, 2‐(2H‐benzotriazol‐2‐yl)‐4,6‐bis(1‐methyl‐1‐phenylethyl)phenol (UV‐234) and 2‐(2H‐benzotriazol‐2‐yl)‐4,6‐di‐tert‐pentylphenol (UV‐328), in juvenile rainbow trout (Oncorhynchus mykiss) chronically exposed (for 28 d) through the diet. Chemical analyses were used to measure liver accumulation of the 2 compounds in exposed trout. Hepatic RNA‐sequencing and qRT-PCR were used to measure gene transcription levels in exposed fish. Results were published in a scientific article that can be found here: https://doi.org/10.1002/etc.4676