Effects of multi-well plate incubation on embryo-larval development in the Fathead minnow (Pimephales promelas)

Fathead minnow (Pimephales promelas) embryos and larvae are frequently used in toxicology, including short-term embryo-only tests which often use small volumes of test solution. The effect that such conditions may have on Fathead minnow development has yet to be explicitly described. Here we compared rates of embryonic development in Fathead minnow embryos reared under standard light and temperature conditions with a range of possible methods. All methods yielded excellent control survival. We demonstrated that Fathead minnow embryos incubated in a range of small volumes in multi-well plates [500 microlitres (μL) to 2 millilitres (mL) per embryo] did not substantially vary in developmental rate, but flexed less frequently as embryos, hatched smaller, later and with larger yolk-sacs, and initiated feeding later than embryos reared in an excess of solution (20 mL per embryo) with or without supplemental aeration. Faster hatch and growth were promoted with an orbital shaker, but growth benefits were not sustained into the larval stage. Developmental differences persisted in larvae reared to 20 days post-fertilization when monitoring ceased, but growth differences did not magnify and in some measurements partially resolved. To our knowledge we are the first to report effects of incubation in multi-well plates in any fish taxa. As our data revealed that the eleutheroembryonic stage for Fathead minnow may be prolonged in multi-well plates, this may allow the use of longer toxicity tests using Fathead minnow embryos without conflicting with existing animal welfare legislation in many countries.


The data file presents the individual length and weight to each Fathead minnow fry at hatch.

Basic view

Metadata Record Information

File Identifier
fc23a03f-c67b-498a-9876-bf96704a8ea7 XML
Date Stamp
2022-11-09T14:39:13
Metadata language

eng; CAN
Character set
UTF8
Hierarchy Level
Dataset
Point of contact
  Government of Canada; Environment and Climate Change Canada; Science and Technology Branch / Water Science and Technology / Aquatic Contaminants Research Division - ACRD Data Management Team
867 Lakeshore Rd. , Burlington , Ontario , L7S 1A1 , Canada
 

Data identification

Date (Publication)
2016-05-17
Date (Creation)
2014-01-01
Status
Completed
Metadata language

eng; CAN
Character set
UTF8
Topic category
  • Environment
  • Biota
Maintenance and Update Frequency
Not planned
Spatial representation type
Text, table
Point of contact
  Government of Canada; Environment and Climate Change Canada; Science and Technology Branch / Water Science and Technology / Aquatic Contaminants Research Division - Dr. Joanne Parrott ( Research Scientist )
867 Lakeshore Rd. , Burlington , Ontario , L7S 1A1 , Canada

Keywords

Theme

  • Observation/Measurement

  • fish embryo toxicity tests

  • embryo

  • bioassay

  • tissue culture plate

  • embryo movement

  • methodology development

  • fish development

  • Chemicals Management Plan (CMP)

  • Contaminants

  • Early-life stage toxicity tests

  • Fish

  • Fathead minnow (Pimephales promelas)

Government of Canada Core Subject Thesaurus

  • Bioassay

  • Fish

ECCC Information Category EN

  • Nature and Biodiversity - Contaminants

Business Functions

  • Assess Toxicity, Manage and Monitor for Environmental Presence of Hazardous Substances and Waste

  • Monitor / Assess Substance and Waste Levels in Air, Water, Soil, Biota

Geography

  • Ontario (ON)

Branch

  • Science and Technology Branch

Directorate

  • Water Science and Technology

Program (PAA)

  • 1.2.1. Water Quality and Aquatic Ecosystems Health

GC Security Level

  • Unclassified
 
Use Limitation

Open Government Licence - Canada ( http://open.canada.ca/en/open-government-licence-canada )

Begin Date
2014-01-01
End Date
2014-12-31

Extent

N
S
E
W
thumbnail


 
 

Ref. system Reference Systems

Reference system identifier
http://www.epsg-registry.org / EPSG:4326 /
 

Distribution

Distribution Formats

Distribution format
  • CSV ( 1 )

 
Distributor
  Government of Canada; Environment and Climate Change Canada - Public inquiries centre
Fontaine Building 12th floor, 200 Sacré-Coeur Blvd. , Gatineau , Quebec , K1A 0H3 , Canada
1-800-668-6767
https://www.canada.ca
 
 

Overviews

N
S
E
W
thumbnail


Keywords

Chemicals Management Plan (CMP) Contaminants Early-life stage toxicity tests Fathead minnow (Pimephales promelas) Fish Observation/Measurement bioassay embryo embryo movement fish development fish embryo toxicity tests methodology development tissue culture plate
Branch
Science and Technology Branch
Business Functions
Assess Toxicity, Manage and Monitor for Environmental Presence of Hazardous Substances and Waste Monitor / Assess Substance and Waste Levels in Air, Water, Soil, Biota
Directorate
Water Science and Technology
ECCC Information Category EN
Nature and Biodiversity - Contaminants
GC Security Level
Unclassified
Government of Canada Core Subject Thesaurus
Bioassay Fish
Program (PAA)
1.2.1. Water Quality and Aquatic Ecosystems Health

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