Effects of PAH containing mixtures on cormorant embryos
The tumor suppressor gene (TP53) is a biomarker of exposure and toxicity of PAHs, or other mutagens and genotoxins. The TP53 pathway regulates gene repair, cellular growth, and apoptosis, following induced genetic damage. We will be using avian (double crested cormorant) models from laboratory egg injection studies to develop these assays. Our goal is to assess the effects of PAH containing mixtures to cormorants in ovo (egg injections), by examining transcriptomics of the TP53 pathway in the embryo. Cormorant eggs will be injected with the PAH mixture, and at pipping. embryos will be sacrificed, genomic DNA extracted from tissues, and sequenced for mRNA to evaluate the TP53 signalling pathway, and the up- or down-regulation of key promoter and regulator genes such as P21, BAX, GADD45, P53R2, and MDM2.
Basic view
Metadata Record Information
- File Identifier
- 925dd208-a3c3-4a84-9bef-475498e1b820 XML
- Date Stamp
- 2021-05-12T06:01:19
- Metadata language
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eng; CAN
- Character set
- UTF8
- Hierarchy Level
- Series
http://ec.gc.ca
Service Identification (19119)
- Date (Creation)
- 2016-09-27
- Date (Publication)
- 2016-09-27
Keywords
- Theme
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PAHs; toxicity; gene expression
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Government of Canada Core Subject Thesaurus
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Toxicology
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ECCC Information Category EN
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Nature and Biodiversity - Contaminants
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Business Functions
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Assess Toxicity of Substance
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Assess Toxicity, Manage and Monitor for Environmental Presence of Hazardous Substances and Waste
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Geography
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Ontario (ON)
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external.theme.EC_Branch
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Science and Technology Branch
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external.theme.EC_Directorate
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Wildlife and Landscape Science
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external.theme.GC_Security_Level
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Unclassified
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Data identification
- Date (Creation)
- 2016-09-27
- Date (Publication)
- 2016-09-27
- Status
- On going
- Metadata language
-
eng; CAN
- Character set
- UTF8
- Topic category
-
- Biota
- Maintenance and Update Frequency
- As needed
- Spatial representation type
- Vector
Keywords
- Theme
-
-
PAHs; toxicity; gene expression
-
-
Government of Canada Core Subject Thesaurus
-
-
Toxicology
-
-
ECCC Information Category EN
-
-
Nature and Biodiversity - Contaminants
-
-
Business Functions
-
-
Assess Toxicity of Substance
-
Assess Toxicity, Manage and Monitor for Environmental Presence of Hazardous Substances and Waste
-
-
Geography
-
-
Ontario (ON)
-
-
external.theme.EC_Branch
-
-
Science and Technology Branch
-
-
external.theme.EC_Directorate
-
-
Wildlife and Landscape Science
-
-
external.theme.GC_Security_Level
-
-
Unclassified
-
- Use Limitation
-
Open Government Licence - Canada ( http://open.canada.ca/en/open-government-licence-canada)
- Access Constraints
- Copyright
- Use Constraints
- Copyright
- Begin Date
- 2016-09-27
Extent
Ref. system Reference Systems
- Reference system identifier
- EPSG / 4326 /
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unknown
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unknown
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http://ec.gc.ca