Experiment 7: Effects of REEs (Nd, Ce) on the aquatic invertebrate Lymnaea stagnalis using eggs, egg masses and juvenile snails – establishing a correction/extrapolation factor

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The objective of this study was to measure the toxicity of two widely used REEs, cerium and neodymium, to the great pond snail Lymnaea stagnalis L. using single eggs, egg masses and juvenile snails. It was expected that snails in individual egg exposure experiments (easier to conduct, but representing a worst case scenario) would be more sensitive than those in either an egg mass or juvenile stage experiment (more difficult to conduct, but more environmentally relevant). For the embryo experiment, eggs were separated from their gelatinous membranes and transferred to wells containing 3 mL of the test solution. Five or six doses were tested and each dose included 48 replicates. The goal of the egg mass experiment was to test the protective ability of the gelatinous membrane surrounding the eggs. All 60 egg masses (ten replicates for six doses) were collected and contaminated within 24 hours of being laid. Juvenile snails were isolated in 2L semi-translucent HDPE plastic containers with 0.5L of test solution. Each container housed one snail, and each dose had eight replicates which were fed organic lettuce, changed three times weekly. All experiments were conducted in a growth chamber (Conviron, model PGW36, Winnipeg, Canada) at 21±2°C, with 16:8h light:dark cycles, under a light intensity of approximately 164 µE m-2s-1. Our studies revealed that single eggs exposed to cerium or neodymium were more sensitive than juveniles and to a lesser extent, egg masses. The unique scientific information presented in these studies will assist regulators in the assessment of the risk related to effects of REEs on invertebrates found in areas of contamination.

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Metadata Record Information

File Identifier
File Identifier
10191eb7-749c-4f4e-a172-6803c1cd7580 XML
Date Stamp
Date Stamp
2021-05-12T06:01:32
Metadata language

eng; CAN
Character set
Character set
UTF8
UTF8
Hierarchy Level
Hierarchy Level
Series
Series
Author
Author
 
Gouvernement du Canada; Environnement et Changement climatique Canada
 - Environment and Climate Change Canada (
Centre de renseignements à la population
)

200 Sacré-Coeur Blvd, Édifice Fontaine 12e étage
, Gatineau,
Québec
, K1A 0H3,
1-800-668-6767

http://ec.gc.ca
 

Data identification

Title

Experiment 7: Effects of REEs (Nd, Ce) on the aquatic invertebrate Lymnaea stagnalis using eggs, egg masses and juvenile snails – establishing a correction/extrapolation factor

Date (Publication)
Date (Publication)
2018-06-20
Date (Creation)
Date (Creation)
2018-06-20
Status
Status
Completed
Completed
Metadata language

eng; CAN
Character set
Character set
UTF8
UTF8
Topic category
Topic category
  • Environment
    Environment
Maintenance and Update Frequency
Maintenance and Update Frequency
As needed
As needed
Spatial representation type
Spatial representation type
Vector
Vector
Principal investigator
Principal investigator
 
Gouvernement du Canada; Environnement et Changement climatique Canada; Science et technologie/Sciences de la faune et du paysage/Division de l'écotoxicologie et de la santé de la faune
- Céline Boutin (
Chercheure scientifique
)

1125 Colonel By Drive, NWRC, Carleton University
, Ottawa,
Ontario
, K1A0H3,
Canada (le)
613-998-0493

613-998-0458

celine.boutin@icloud.com

Keywords

Theme
Theme

  • Terres rares, REEs, Cerium, Neodymium, Escargot aquatique, test de toxicité

  • Substance

Thésaurus des sujets de base du gouvernement du Canada

  • Toxicologie

ECCC Information Category FR

  • Nature et biodiversité - contaminants

Fonctions de l'entreprise

  • Évaluer la toxicité de la présence dans l'environnement des substances et des déchets dangereux, les gérer et les surveiller

  • Évaluer la toxicité de la substance

Portée Géographique

  • National (CA)

local.theme.external.theme.EC_Branch

  • Direction générale des sciences et de la technologie

local.theme.external.theme.EC_Directorate

  • Sciences de la faune et du paysage

local.theme.external.theme.EC_Program_PAA

  • 1.1 Biodiversité - Espèces sauvages et habitats

local.theme.external.theme.GC_Security_Level

  • Confidentiel
 
Use Limitation

Licence du gouvernement ouvert - Canada ( http://ouvert.canada.ca/fr/licence-du-gouvernement-ouvert-canada)

Access Constraints
Access Constraints
License
License
Use Constraints
Use Constraints
License
License
Begin Date
2018-06-20

Extent

N
S
E
W
thumbnail




 
 

Ref. system Reference Systems

Reference system identifier
Reference system identifier
EPSG / 4326 / Inconnue
 

Distribution

Distribution Formats

Distribution format
Distribution format
  • CSV ( CSV)

 
Distributor
Distributor
 
Gouvernement du Canada; Environnement et Changement climatique Canada
 - Environment and Climate Change Canada (
Centre de renseignements à la population
)

200 Sacré-Coeur Blvd, Édifice Fontaine 12e étage
, Gatineau,
Québec
, K1A 0H3,
1-800-668-6767

http://ec.gc.ca
 
 

Overviews

N
S
E
W
thumbnail




Keywords

Substance
Terres rares, REEs, Cerium, Neodymium, Escargot aquatique, test de toxicité
ECCC Information Category FR

Nature et biodiversité - contaminants
Fonctions de l'entreprise

Évaluer la toxicité de la présence dans l'environnement des substances et des déchets dangereux, les gérer et les surveiller
Évaluer la toxicité de la substance
Thésaurus des sujets de base du gouvernement du Canada

Toxicologie
local.theme.external.theme.EC_Branch

Direction générale des sciences et de la technologie
local.theme.external.theme.EC_Directorate

Sciences de la faune et du paysage
local.theme.external.theme.EC_Program_PAA

1.1 Biodiversité - Espèces sauvages et habitats
local.theme.external.theme.GC_Security_Level

Confidentiel


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