Experiment 7: Effects of REEs (Nd, Ce) on the aquatic invertebrate Lymnaea stagnalis using eggs, egg masses and juvenile snails – establishing a correction/extrapolation factor

The objective of this study was to measure the toxicity of two widely used REEs, cerium and neodymium, to the great pond snail Lymnaea stagnalis L. using single eggs, egg masses and juvenile snails. It was expected that snails in individual egg exposure experiments (easier to conduct, but representing a worst case scenario) would be more sensitive than those in either an egg mass or juvenile stage experiment (more difficult to conduct, but more environmentally relevant). For the embryo experiment, eggs were separated from their gelatinous membranes and transferred to wells containing 3 mL of the test solution. Five or six doses were tested and each dose included 48 replicates. The goal of the egg mass experiment was to test the protective ability of the gelatinous membrane surrounding the eggs. All 60 egg masses (ten replicates for six doses) were collected and contaminated within 24 hours of being laid. Juvenile snails were isolated in 2L semi-translucent HDPE plastic containers with 0.5L of test solution. Each container housed one snail, and each dose had eight replicates which were fed organic lettuce, changed three times weekly. All experiments were conducted in a growth chamber (Conviron, model PGW36, Winnipeg, Canada) at 21±2°C, with 16:8h light:dark cycles, under a light intensity of approximately 164 µE m-2s-1. Our studies revealed that single eggs exposed to cerium or neodymium were more sensitive than juveniles and to a lesser extent, egg masses. The unique scientific information presented in these studies will assist regulators in the assessment of the risk related to effects of REEs on invertebrates found in areas of contamination.

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Metadata Record Information

File Identifier
10191eb7-749c-4f4e-a172-6803c1cd7580 XML
Date Stamp
2021-05-12T06:01:32
Metadata language

eng; CAN
Character set
UTF8
Hierarchy Level
Series
Author
  Government of Canada; Environment and Climate Change Canada - Environment and Climate Change Canada ( Public inquiries centre )
Fontaine Building 12th floor, 200 Sacré-Coeur Blvd , Gatineau , Quebec , K1A 0H3 ,
1-800-668-6767
http://ec.gc.ca
 

Data identification

Date (Publication)
2018-06-20
Date (Creation)
2018-06-20
Status
Completed
Metadata language

eng; CAN
Character set
UTF8
Topic category
  • Environment
Maintenance and Update Frequency
As needed
Spatial representation type
Vector
Principal investigator
  Government of Canada; Environment and Climate Change Canada; Science & Technology/Wildlife and Landscape Science/Ecotoxicology and Wildlife Health Division - Céline Boutin ( Research Scientist )
1125 Colonel By Drive, NWRC, Carleton University , Ottawa , Ontario , K1A0H3 , Canada
613-998-0493
613-998-0458
celine.boutin@icloud.com

Keywords

Theme

  • Rare Earth Elements, REEs, Cerium, Neodymium, Aquatic snail, toxicity test

  • Substance

Government of Canada Core Subject Thesaurus

  • Toxicology

ECCC Information Category EN

  • Nature and Biodiversity - Contaminants

Business Functions

  • Assess Toxicity, Manage and Monitor for Environmental Presence of Hazardous Substances and Waste

  • Assess Toxicity of Substance

Geography

  • National (CA)

external.theme.EC_Branch

  • Science and Technology Branch

external.theme.EC_Directorate

  • Wildlife and Landscape Science

external.theme.EC_Program_PAA

  • 1.1. Biodiversity – Wildlife and Habitat

external.theme.GC_Security_Level

  • Confidential
 
Use Limitation

Open Government Licence - Canada ( http://open.canada.ca/en/open-government-licence-canada )

Access Constraints
License
Use Constraints
License
Begin Date
2018-06-20

Extent

N
S
E
W
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Ref. system Reference Systems

Reference system identifier
EPSG / 4326 / Inconnue
 

Distribution

Distribution Formats

Distribution format
  • CSV ( CSV )

 
Distributor
  Government of Canada; Environment and Climate Change Canada - Environment and Climate Change Canada ( Public inquiries centre )
Fontaine Building 12th floor, 200 Sacré-Coeur Blvd , Gatineau , Quebec , K1A 0H3 ,
1-800-668-6767
http://ec.gc.ca
 
 

Overviews

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S
E
W
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Keywords

Rare Earth Elements, REEs, Cerium, Neodymium, Aquatic snail, toxicity test Substance
Business Functions
Assess Toxicity of Substance Assess Toxicity, Manage and Monitor for Environmental Presence of Hazardous Substances and Waste
ECCC Information Category EN
Nature and Biodiversity - Contaminants
Government of Canada Core Subject Thesaurus
Toxicology
external.theme.EC_Branch
Science and Technology Branch
external.theme.EC_Directorate
Wildlife and Landscape Science
external.theme.EC_Program_PAA
1.1. Biodiversity – Wildlife and Habitat
external.theme.GC_Security_Level
Confidential

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